Abstract

ANALYTICAL METHODS FOR ESTIMATION OF DIDANOSINE: A REVIEW

Md. Zameeruddin, Kalyankar Swapnil S*., Jadhav S.B., Kadam V.S., Bharkad V.B. andSolanke S.B.

ABSTRACT

Antiretroviral (ARV) therapy is potent, convenient and usually well tolerated, capable of reducing HIV blood concentration to undectable values within a few weeks from treatment initiation and of inducing a robust and sustained cluster of differentiation antigen (CD4 T-cell) gain. Didanosine (DDI) metabolized intracellularly by series of cellular enzymes to its active moiety, dideoxyadenosine triphosphate, which inhibits the HIV reverse transcriptase enzymes competitively with natural deoxyadenosine triphosphate (DATP). It is white not hygroscopic crystalline powder having melting point 160-163°C, water soluble i.e., (27.3 mg/mL at 25°C and pH 6.2), soluble in dimethylsulfoxide, slightly soluble in ethanol and methanol, insoluble in chloroform. A simple, specific, accurate, economical and precise reversed phase high performance liquid chromatography (RP-HPLC) and UV spectrophotometric method has been developed for the simultaneous estimation and absorption ration method. The method was validated for Linearity, Specificity, Accuracy, Precision, Ruggedness, Robustness, Limit of Detection (LOD) and Limit of Quantification (LOQ). This review chromatographic separation was performed Chromosil column, Lochrospher 100 RP-8, C18 column, C18 catridges with distilled water, methanol, ethanol, acetonitrile, phosphate buffer etc.

Keywords: UV spectrophotometric, RP-HPLC, Didanosine, Validation.