Abstract

ISOLATION AND CHARACTERIZATION OF ACID-SOLUBILIZED COLLAGEN FROM THE SKIN OF FRESHWATER SNAKEHEAD FISH CHANNA STRIATUS”

A. Palanivel, M. Prakash*, G. Gunasekaran and M. Rajesh

ABSTRACT

Acid-Solubilized Collagen was extracted from the skin of freshwater snakehead fish Channa striatus and characterized (SDS-PAGE, Amino acid, FT-IR study, Solubility). The SDS-PAGE patterns were identified to be the type-I collagen with major components of α1, α2 and β chains. The characterization of amino acid showed that glycine occupies more when compared to other acids followed by alanine, proline, hydroxyproline and glutamine. The FT-IR study shows the presence of the all the three amides (Amide I, II and III) with their respective frequencies.

Keywords: Channa striatus, snakehead fish, Acid solubilized collagen, type – I collagen.