Abstract

DEVELOPMENT OF ELISPOT FOR RAPID DETECTION OF ROTAVIRUS INFECTION USING AVAIN IGY ANTIBODIES

DEVELOPMENT OF ELISPOT FOR RAPID DETECTION OF ROTAVIRUS INFECTION USING AVAIN IGY ANTIBODIES

ABSTRACT

Immunoassays are widely being used globally for the detection of rotavirus infection. Keeping pace with the severety of infections due to the development of various strains, detection assays are also developed to identify the infection rate rapidly. A strip based ELISA assay, ELISPOT was developed for rapid detection of rotavirus infection and to determine the specificity and sensitivity of anti-recRVP6 IgY antibodies using western blotting assay. The limit of detection was 1μl/ml and the quantification was 1μg/ml of concentration was sufficient for the strip based ELISA detection. This asssay is quite handy and easily adoptable having higher sensitivity than currently available tests for rotavirus detection.

Keywords: ELISPOT, IgY antibodies, rapid detection, western blotting, sensitivity.