Abstract

NON VIRAL SYNTHETIC siRNA DELIVERY SYSTEM AN EFFICIENT TOOL FOR CANCER TREATMENT

Gajendra J C Gupta*, Priti P Pednekar, K R Jadhav, Sai V Chilajwar,Vilasrao J Kadam

ABSTRACT

RNA interference (RNAi) is an evolving conserved cellular process for the regulation of gene expression for the treatment of cancer. RNAi is induced by short (21-23 nt) duplexes of RNA, termed as short interference RNA (siRNA) that can elicit highly sequence specific gene silencing.Although several siRNA candidates for the treatment of ocular and respiratory diseases are still undergoing clinical trials, but some inherent challenges needed for the further development of siRNAs for anti-cancer therapeutics, because systemic administration will be required in most cases. In addition to nonspecific off target and immune stimulation problems, appropriate dose delivery remains a major hurdle. Synthetic siRNA are poly anionic macromolecules that do not readily enter the cell and thus typically requires delivery system for effective gene silencing.This review is intended to give the insight about the various Non-viral delivery strategies including their characterization, chemical modification, conjugation, lipid-based techniques, polymer-based nanosystems, and physical methods. Moreover, the current progress in siRNA delivery systems for cancer treatment is further discussed.

Keywords: Gene expression, Gene silencing, Off target, Polyanionic macromolecules, RNA interference, siRNA