CHARACTERIZATION OF BACTERIOCIN PRODUCTION FROM A NEW LACTIC ACID BACTERIA
*Ankita Rajvanshi1, Vinod Kumar Nigam 3, Shobha Kulshreshtha2 and Ram Prayag Pareek1
1Department of Biotechnology, NIMS University, Jaipur, Rajasthan, India.
2Department of Pharmacology, NIMS University, Jaipur, Rajasthan, India.
3Department of Biotechnology, Birla Institute of Technology, Mesra Ranchi, Jharkhand, India.
ABSTRACT
A strain of Lactic Acid Bacteria isolated from cucumber collected
from Jaipur city, India was screened for bacteriocin production and
characterization. The antibacterial compound produced by the isolate
showed antagonistic activity against human pathogenic bacteria such
as Escherichia coli, Salmonella typhi and Staphylococcus aureus. The
antibacterial compound was resistant at acidic pH and able to tolerate
high concentration of sodium chloride and bile salts.
Bacteriocin producing strain was identified on the basis of
phenotypic, various biochemical tests and 16S rDNA sequence as
Leuconostoc mesenteroides MTCC 10508. Based on the several
characteristics of the bacteriocin produced from Leuconostoc
mesenteroides, it has been observed that the strain can act as Probiotic
agent to improve the microbial quality and safety of fresh vegetables
and fruits.
Keywords: Antagonism, Bacteriocin, Cucumber, Lactic Acid Bacteria, Probiotic.
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