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Abstract

CHARACTERIZATION OF BACTERIOCIN PRODUCTION FROM A NEW LACTIC ACID BACTERIA

*Ankita Rajvanshi1, Vinod Kumar Nigam 3, Shobha Kulshreshtha2 and Ram Prayag Pareek1

1Department of Biotechnology, NIMS University, Jaipur, Rajasthan, India.
2Department of Pharmacology, NIMS University, Jaipur, Rajasthan, India.
3Department of Biotechnology, Birla Institute of Technology, Mesra Ranchi, Jharkhand, India.

ABSTRACT

A strain of Lactic Acid Bacteria isolated from cucumber collected from Jaipur city, India was screened for bacteriocin production and characterization. The antibacterial compound produced by the isolate showed antagonistic activity against human pathogenic bacteria such as Escherichia coli, Salmonella typhi and Staphylococcus aureus. The antibacterial compound was resistant at acidic pH and able to tolerate high concentration of sodium chloride and bile salts. Bacteriocin producing strain was identified on the basis of phenotypic, various biochemical tests and 16S rDNA sequence as Leuconostoc mesenteroides MTCC 10508. Based on the several characteristics of the bacteriocin produced from Leuconostoc mesenteroides, it has been observed that the strain can act as Probiotic agent to improve the microbial quality and safety of fresh vegetables and fruits.

Keywords: Antagonism, Bacteriocin, Cucumber, Lactic Acid Bacteria, Probiotic.


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