Abstract

EXTRACTION, IDENTIFICATION AND ANTIFUNGAL STUDIES OF LEAVES EXTRACT OF PUNICA GRANATUM L.

Ibrahim.S.Abaas* and Mohammed.J.Hamzah, Ali.J.Ali

ABSTRACT

Leaves samples of pomegranate were collected from medicinal plants Garden of College of pharmacy of Kerbela University, the detection results of activecompounds were referred to pomegranate leaves were contain tannins, glycosides, phenols, saponnines, resin, alkaloids, comurins and flavones. Thirty five samples were collected from patients (6-30)years old, suffered skin, rushes, boils oral thrush, anal and vaginal itches. Candida albicans 57.3% (20 isolates ) and Candida tropicalis 22.5% (8 isolates ) Aspergills fumegatus 11.5 % (4 isolates )Aspergills nigra8.7%( 3 isolates ), were isolated and identified from these samples .Alcoholic and water hot extracts of the pomegranate leaves as well as the dried powder were prepared .The anti-fungal activity of the extracts was evaluated by means of agar – well diffusion assay. The extract exhibited potent activity against yeast. The minimum inhibitory concentration were 128- 1024 mg/ml against Cndida albicans and Candida tropicalis .There was little difference between the activities of alcoholic extract and aqueous extract .These results suggest the Pomegranate leaves extract which contains gallotanic acid as a promising anti – fungal agent.

Keywords: Extraction, Identification,antifungal activity, Pome.