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Abstract

ANTIMICROBIAL ACTIVITY OF PENICILLIUM MINIOLUTEUM ED24, AN ENDOPHYTIC FUNGUS RESIDING IN ORTHOSIPHON STAMINEUS BENTH

Tong Woei Yenn*, Ang Swee Ngim, Darah Ibrahim and Latiffah Zakaria

ABSTRACT

The isolate ED24 was previously isolated as endophytic fungus residing in medicinal herb Orthosiphon stamineus Benth. The isolate was identified as Penicillium minioluteum based on its macroscopic and microscopic features. The result was further confirmed by molecular identification. On disc diffusion assay, the isolate exhibited significant inhibitory activity of various test bacteria. However, none of the test yeasts and test fungi were inhibited by the isolate. The crude extracts prepared from fungal biomass of Penicillium minioluteum ED24 was found to reported better antibacterial activity than the fermentative broth. The antibacterial activities of the extracts are concentration dependent and the MIC values ranged from 15.6 to 500 μg/mL. The wide range of MIC values indicates the different susceptibility level of the test microorganisms to the fungal extract.

Keywords: Orthosiphon stamineus, MIC, Penicillium minioluteum


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