Abstract

ANTIHYPERGLYCEMIC, ANTIHYPERLIPIDEMIC EFFECTS OF ETHANOL EXTRACTS OF NIGELLA SATIVA AND APIUM GRAVEOLENS AND THEIR COMBINATION IN STREPTOZOCIN/ HIGH FAT DIET INDUCED HYPERGLYCEMIC MICE

*Alaa Hamzha Abbas

ABSTRACT

Objective: The present study was designed to determine the antihyperglycemic and antihyperlipidemic effects of ethanol extract of Nigella sativa, Apium Graveolens and their combination in Streptozocin/ high fat diet induced hyperglycemic mice. Materials & methods: The normal serum values of glucose were determined in 60 healthy male mice before Streptozocin/ high fat diet induction & at two occasions 14,28 day after induction by Streptozocin/ high fat diet and treatment with ethanolic extract of Nigella sativa, Apium Graveolens and their combination for 28 days, also serum C- peptide, MDA, GSH, cholesterol, triglyceride, vLDL, LDL, HDL are measured at end of experiment. Results: Nigella sativas, Apium Graveolens and their combination showed decrease in serum glucose, cholesterol, triglyceride, vLDL, LDL, also increase in serum C-peptide and serum HDL level measured at day 28 compared with glyburide group, also there is improvement in MDA and GSH serum levels but better results obtained with treatment of combination of the two herbs. Conclusion: Ethanol extracts of Nigella sativa, Apium Graveolens and their combination posses mild Antihyperglycemic, Antihyperlipidemic effect however, the combination of two herbs extract showed the best results. The histopathological examination showed improvement in the sections of the pancreas tissue that support the effect of extract on the pancreas.

Keywords: Nigella sativa, Apium Graveolens, Antihyperglycemic, Antihyperlipidemic, STZ, HFD.