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Abstract

ASSAYS OF DOXYCYCLINE ANTIBIOTIC USED IN TASIKMALAYA CITY HEALTH CENTER FOR EVALUATION CAUSE ANTIBIOTIC RESISTANCE

Danni Ramdhani* and Resmi Mustarichie

ABSTRACT

An acute respiratory tract infection (ARIs) is a disease attacking one or more parts of the respiratory tract. ARI is mostly caused by bacteria than viruses. For treatment of ARI, people tend to give some antibiotics. But there are many cases of bacterial resistance to some antibiotics have been reported. Testing levels of antibiotics is one of the evaluation to determine the quality of antibiotics used. This quality evaluation to determine the role of antibiotics in causing antibiotic resistance. Method: Samples were obtained from Tasikmalaya City Health Department. Samples derived from only one pharmaceutical factory in Indonesia, with one production batch number. Tests carried out using UV-Visible spectrophotometer instrument, with validation parameters: linearity, precision, accuracy, limit of detection (LOD) and limit of Quantification (LOQ). Result: The test results showed that the levels of doxycycline antibiotics that are used to meet the requirements that exist in the Indonesian Pharmacopoeia grading 115,16%, and the validation parameters that meet the requirements, namely koefesisen correlation of 0.9969, reproducibility 0,01013%, percent recovery 99,827%, LOD 5,810, and LOQ 19,368.

Keywords: Doxycycline, UV-Visible Spectrophotometer, Validation Parameters, Indonesia Pharmacopoeia.


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