Abstract

EFFECT OF pH ON AMINOFUNCTIONALIZED MESOPOROUS SILICA NANOPARTICLES LOADED WITH 5-FLUOROURACIL AND ITS OPTIMIZATION

Dr. Kala D.*, Jijo Thomas and Deepa V. Nair

ABSTRACT

The purpose of this study was to determine the effect of pH on loading of 5-Fluorouracil (FU) on amino functionalized mesoporous silica nanoparticles. Loading was done by backfilling approach. Optimization is carried out by using Box Behnken design. Independent variables were 5- FU concentration in pH 8 buffer (A), 5-FU: APT-MSN (aminofunctionalized mesoporous silica nanoparticle) 1:1 ratio (B), incubation time (C). The selected dependent variables include drug entrapment efficiency (R1), drug loading efficiency (R2). Amino functionalization enhances 5-FU loading on mesoporous silica nanoparticles. pH 8 buffer was found to be optimum with a maximum entrapment efficiency of 75.47% for 1:1 ratio of APT-MSN:5-FU. The drug release kinetics exhibited by 5-FU: APT-MSN found to follow Higuchi model with non-fickian anomalous diffusion mechanism.

Keywords: Amino functionalized mesoporous silica nanoparticle, 5-Fluorouracil, Box –Behnken design, entrapment efficiency, loading efficiency.