Abstract

EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF POLYHERBAL FORMULATION AGAINST ETHANOL INDUCED HEPATOTOXICITY IN RATS

B. Sreshta* and S. Ravindra Babu

ABSTRACT

The objective of the present study was to assess the hepatoprotective effect of polyherbal formulation(PHF) in ethanol induced hepatotoxicity in rats. Hepatoprotective activity of the PHF containing two indigenous medicinal plant extracts Tinospora cordifolia and Curculigo orchioides, was screened against ethanol induced hepatotoxicity in rats. PHF was evaluated by measuring levels of serum marker enzymes like SGOT, SGPT, albumin, creatinine and total protein. The histological studies were also studied to support the biochemical parameters. Silymarin was used as standard drug. Administration of ethanol shows significant morphological, biochemical and histopathological deteriorations in the liver of experimental animals. Administration of PHF (200 and 400 mg/kg p.o.) significantly inhibited(p<0.05) ethanol-induced elevation levels of SGOT, SGPT, albumin, creatinine and significantly increased(p<0.05) levels of total protein content. A comparative histopathological study of liver exhibited almost normal architecture as compared to toxicant group. Results suggests that the hepatoprotective effects of PHF might be useful for liver protection due to combined action of all plant extracts along with their phytoconstituents.

Keywords: Poly herbal formulation, Hepatotoxicity, Ethanol, CCl4, Tinospora cordifolia, Curculigo orchioides.