WJPPS Citation

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • New Impact Factor
  • WJPPS Impact Factor has been Increased to 8.025 for Year 2024.

  • WJPPS: MARCH ISSUE PUBLISHED
  • March Issue has been successfully launched on 1 March 2024.

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • Scope Indexed
  • WJPPS is indexed in Scope Database based on the recommendation of the Content Selection Committee (CSC).

Abstract

METHOD DEVELOPMENT AND VALIDATION OF RP – HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DAUNORUBICIN AND CYTARABINE IN SYNTHETIC MIXTURE

*Rajvanshi Kushagra

ABSTRACT

A simple, precise, specific, accurate and robust RP-HPLC method has been developed & validated as per ICH guideline Q2 R(1) for the simultaneous determination of Cytarabine and Daunorubicin. Chromatographic separation of these two drugs were carried out on C18 column of Inertsil (150mm x 4.6mm x 3.4μm) as stationary phase with a mobile phase consisting of Methanol:phosphate Buffer (3pH) (60:40%v/v) at a flow rate of 1mL/min and UV detection at 238 nm. The retention time of Cytarabine and Daunorubicin were 2.306 min and 4.342 min respectively. The proposed method was validated for specificity, linearity, accuracy, precision, LOD & LOQ. The describe method was linear over a concentration range of 5-15 μg/mL & 2.2 – 6.6 μg/mL for Cytarabine and Daunorubicin respectively. The % recoveries of Cytarabine and Daunorubicin were found 100.29% - 102.23% and 98.62% - 102.59%. respectively. The optimized and validated method can be used for estimation of Cytarabine and Daunorubicin in bulk and also in Synthetic mixture.

Keywords: Cytarabine, Daunorubicin, RP-HPLC, Validation, Synthetic mixture.


[Download Article]     [Download Certifiate]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More