Abstract

IN VITRO STUDY OF THE ANTICOAGULANT ACTIVITY OF SOME PLANT EXTRACTS

Kartheek Chegu*, K. Mounika, M. Rajeswari, N. Vanibala, P. Sujatha, P. Sridurga, D. R. Brahma Reddy

ABSTRACT

Hemostasis is the process of formation of clots within the walls of damaged blood vessels. To prevent abnormal bleeding and to maintain intravascular blood in a fluid state, in this study we aimed to evaluate the possible anticoagulant effect of aqueous extracts of Ginger, Garlic, Green tea and Clove. The aqueous extracts of Ginger, Garlic, Green tea and Clove were tested for in vitro prothrombin time (PT) test. The in vitro anticoagulant effects examined by using plasma, collected from blood samples of normal individuals by measuring PT. Ethylenediaminetetraacetic acid (EDTA) and saline in distilled water were used as a negative and positive control, respectively. The extract plasma was subjected to anticoagulation activity and was compared with EDTA-plasma and saline plasma. The observed prolonged prothrombin activity could be due to the presence of certain phytochemical constituents in the crude extract. The crude extracts and further, the active principles could be isolated and evaluated for clinical or physiological purposes. In vitro, anticoagulant activity studies results demonstrated that the all four aqueous extracts possess pharmacologically active anticoagulant components which could be helpful in preventing blood clot.

Keywords: Anticoagulant, Hemostasis, Prothrombin time, Ethylenediaminetetraacetic acid (EDTA).