Abstract

FUNCTIONAL AND PHYLOGENETIC ANALYSIS OF NOD2 GENE WHICH IS RESPONSIBLE FOR CROHN’S DISEASE

Komala S.*, Shoba K. and Nithya G.

ABSTRACT

Crohn's disease is a type of Inflammatory bowel disease (IBD) that may affect any part of the gastrointestinaltract from mouth to anus. The cause of Crohn's disease is believed to be due to a combination of environmental, immune, and bacterial factors in genetically susceptible individuals. It results in a chronic inflammatory disorder, in which the body's immune system attacks the gastrointestinal tract possibly directed at microbial antigens. Nod2 is an intracellular bacterial sensor and its mutations are associated with the development of CD. Nucleotide and Protein sequence for the gene NOD2 is retrieved from National Centre for Biotechnology Information in fasta format. Functional analysis is done by using the AMIGO tool. The gene expression studies were analyzed using insilico tool ACUA. Highly expressed genes is selected on the basis of CAI value. Phylogenetic analysis of target gene is done through using phyML. From the results that the identified conserved motif sequences are the potential protein target sequences for structure based drug designing and molecular drug docking studies. This work would definitely be useful in the field of Clinical Pathology, Computational Entomology and Cheminformatics.

Keywords: Crohn, NOD2. Gene expression, CAI, phylogenetic analysis.