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Abstract

SPECTROPHOTOMETRIC DETERMINATION OF NORFLOXACIN USING CHROME AZUROL S

Malek Okdeh* and Kholoud Kassab

ABSTRACT

An accurate, simple, fast and cheap spectrophotometric method has been developed for the determination of norfloxacin in pharmaceutical pure and dosage forms. The method is based on the reaction of the drug with chrome azurol S in citrate buffer at pH=4. This reaction produces a complex purple colored product which absorbs maximally at 518 nm. Beer’s law was obeyed in the range of 6.38-63.86μg/mL with molar absorptivity of 0.354 × 104 L mole-1cm-1and the correlation coefficient R2 =1. The effects of analytical parameters on the reported system were investigated. The results were validated statistically. The proposed method was applied to commercially available tablets. Interferences of the other ingredients and excipients were not observed.

Keywords: Norfloxacin, Chrome azoul S, Complex formation, Spectrophotometry.


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