Abstract

EFFECT OF PHYSIOCHEMICAL PARAMETERS ON FIBRINOLYTIC PROTEASE PRODUCTION BY SOLID STATE FERMENTATION

E. Venkata Naga Raju*, Divakar Goli

ABSTRACT

This work has been undertaken tostudy the different physiochemical parameters such as various substrates, initial moisture level,substrate particle size, inoculum size, pH, incubation temperature, incubation period, carbon and nitrogen sources effecton the production of fibrinolytic protease in solid-state fermentation using mutant Bacillus cereus GD55. The fibrinolytic protease production was observed with apple pomace (34.28 ± 0.21U/g), initial moisture level 70% (38.39 ± 0.10 U/g),substrate particle size 1.2 mm (42.15 ± 0.17U/g), inoculum size 2.0% (46.86 ± 0.98 U/g),initial pH 8 (46.60 ± 0.36 U/g), incubation temperature 37°C (46.78 ± 0.19U/g),incubation period 3 Days (48.32 ± 0.24U/g), carbon source fructose (52.19 ± 0.13 U/g), peptone (56.18 ± 0.21U/g), NH4NO3 (53.24 ± 0.64 U/g) in the production medium.

Keywords: Physicochemical parameters,Solid-state fermentation, Bacillus cereus GD55, fibrinolytic protease.