Abstract

MEDIA OPTIMIZATION FOR DOCOSAHEXAENOIC ACID PRODUCTION BY SCHIZOCHYTRIUM SPECIES USING STATISTICAL TECHNIQUE

Umesh Luthra*, Archana Tripathi, Sneha Khadpekar, Harish Kumar, Nishtha Kulshreshtha and Aditi Trivedi

ABSTRACT

Many optimization techniques are available for the optimization of fermentation media. Each optimization technique has its own advantages. In this study, Plackett-Burman design and full factorial design are used to optimize the media for DHA production. The effect of various media components such as dextrose, yeast extract, tryptone, NaCl, MgSO4, CaCl2, KH2PO4, KCl were examined. Dextrose, yeast extract, tryptone and NaCl were selected as significant factors by Plackett-Burman design which was further screened by full factorial design. A central composite design and response surface methodology were utilized to optimize the screened factors and to increase the productivity of DHA. A maximum DHA production was achieved 4.48 mg/g (free DHA) with the combination of optimized factors: dextrose 74.92 g/l, tryptone 10.94 g/l and NaCl 5.97 g/l. Validation experiments were also performed to verify the adequacy and accuracy of the model.

Keywords: Schizochytrium species, Docosahexaenoic acid, Submerged Fermentation, Plackett- Burman (PB), Full factorial (FF), Response Surface Methodology (RSM), Central composite design (CCD).