Abstract

PLATELET COUNT INCREASING ACTIVITY AND OTHER BIOLOGICAL STUDIES ON LEAVES OF CARICA PAPAYA

Renuka Itani*, Prof. Dr. Dharma Prasad Khanal and Nisha Shrestha

ABSTRACT

Aim of this study is to explore the medicinal values of plant papaya which will be very effective and becomes practically application. Fresh leaf juice of Carica papaya was used for platelet count increasing activity testing whereas Soxhlet extracted hexane, ethyl acetate and methanolic extracts of leaves Carica papaya were subjected to other biological screening (Brine shrimp lethality bioassay, anti-hyperglycemic effect evaluation and anti-inflammatory effect evaluation). Fresh juice of Carica papaya leaves concentrations of 1g/kg and 2g/kg were found to potentially increase the platelet count in cyclophosphamide induced rat model. The brine shrimp lethality bioassay showed that the hexane extract of Carica papaya was mildly toxic to Artemia salina naupalii having LC50 values 50.188μg/ml. The ethyl acetate and methanolic extracts were found to be non-toxic. The results demonstrate that the methanolic extract possesses anti-hyperglycemic potential. The highest blood glucose lowering effect was observed in 180 minutes of standard drug (32.52%) as well as of Carica papaya extracts 200mg/kg (45.35%) and 400 mg/kg (59.0%). The present study revealed that the test extracted compound showed significant anti-inflammatory activity. The maximum inhibition activity was observed during 5hour i. e. 31.74% of standard, 29.91 of test (200mg/kg) and 31.78% of test (400mg/kg).

Keywords: Platelet count, anti-hyperglycemic, anti-inflammatory.