Abstract

FLAVONOID-RICH EXTRACT OF SOLANUM MACROCARPON LEAVES SHOWS TISSUE PROTECTIVE AND AMELIORATE CERTAIN INFLAMMATORY AND OXIDATIVE INDICES ASSOCIATED WITH D-GALACTOSE EXPOSED RATS

Seyi Elijah Elasoru,*, Olaniyi Temitope Adedosu, John Olabode Fatoki and Busuyi David Kehinde

ABSTRACT

The present study assessed the protective potentials of Flavonoid-Rich Extract of Solanum macrocarpon Leaves (FRESML) against D-galactose-induced oxidative stress in rats. Animals were randomly selected into four groups of seven per group and treated for 28 days. Group A (control) were given distilled water, Group B (extract only) were administered 100mg/kg/day of FRESML, Group C (D-galactose only) were administered 500mg/kg/day of D-galactose and Group D (D-galactose + extract) received combined administration of 500mg/kg/day of D-galactose and 100mg/kg/day of FRESML. Total Protein (TP), Urea and Creatinine concentrations, Alkaline Phosphatase (ALP), Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Gamma Glutamyl Transaminase (GGT), Superoxide Dismutase (SOD) and Catalase activities, Reduced Glutathione (GSH), Malondialdehyde (MDA), Packed Cell Volume (PCV), White Blood Cell (WBC) and Fibrinogen levels were assessed using standard assay methods. D-galactose administration in rats triggered significant (p<0.05) increases in serum levels of ALT, AST, GGT, ALP, Urea and Creatinine and significant (p<0.05) decreases in serum, liver and kidney TP concentrations. Significant (p<0.05) increases in percentage PCV, WBC counts and Fibrinogen concentration were obtained. In addition, significant (p<0.05) increases in liver and kidney MDA concentrations and depletion in liver and kidney SOD, Catalase and GSH levels were also obtained. Combined administration of D-galactose and FRESML significantly (p<0.05) modulated the levels of ALT, AST, GGT, ALP, Urea and Creatinine and significantly (p<0.05) increased serum, liver and kidney TP to nearly control levels. The extract significantly (p<0.05) reversed D-galactose-induced increases in percentage PCV, WBC counts, Fibrinogen and MDA concentration and increases liver and kidney SOD, Catalase and GSH to nearly control level. Group treated with the extract alone showed no adverse effects on the parameters. Results are possible indication of FRESML ability to protect against cellular oxidative stress in tissues, boost antioxidant status and ameliorate inflammation associated with oxidative stress.

Keywords: Antioxidant, D-galactose, Oxidative Stress, Protective, Solanum macrocarpon.