PHYTOCHEMICAL INVESTIGATION AND IN – VITRO FREE RADICAL SCAVANGING ACTIVITY OF MENTHA PULEGIUM CONTAINING PHENOLIC COMPOUNDS
Sachin Jain*1, Aakash Singh Panwar1, Pritesh Paliwal1, Praveen Sharma1
1College of Pharmacy, IPS Academy, Indore 452012 India.
ABSTRACT
To evaluate the in-vitro free radical scavenging activity of leaves
Mentha pulegium linn. Petroleum ether, ethanol, aqueous extracts of
Mentha pulegium were prepared, with successive extraction in soxhlet
apparatus. Each extract was selected to study the free radical
scavenging activity by superoxide scavenging assay method. It was
found that aqueous extract contained carbohydrates, glycosides amino
acids flavonoids, tannins, alkaloids, steroids; ethanolic extract
contained glycosides amino acids flavonoids, tannins, alkaloids,
steroids. Ethanolic extract of Mentha pulegium has showed 58.7±0.62
% inhibition in superoxide scavenging model. Aqueous extract also
showed almost similar activity (54.9±0.53 % compared to ethanolic
extract), while Petroleum ether extract showed poor inhibition of
superoxide scavenging activity. All extracts showed dose and time
dependent inhibition of superoxide scavenging activity. Jatropha
gossypifolia had the highest total phenolic content (42.60 mg TAE
(tannic acid equivalent)/ 100 g fresh weight). Total phenolic content had positive correlation
with antioxidant capacity. This shows that the plants, especially Mentha pulegium, may be
potent source of natural antioxidants.
Keywords: Mentha pulagium, DPPH, Phenolic content, Superoxide scavenging, Antioxidant activity.
[Download Article]
[Download Certifiate]