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  • SEPTEMBER 2020 Issue has been successfully launched on 1 September 2020.

Abstract

PHYTOCHEMICAL INVESTIGATION AND IN – VITRO FREE RADICAL SCAVANGING ACTIVITY OF MENTHA PULEGIUM CONTAINING PHENOLIC COMPOUNDS

Sachin Jain*1, Aakash Singh Panwar1, Pritesh Paliwal1, Praveen Sharma1

1College of Pharmacy, IPS Academy, Indore 452012 India.

ABSTRACT

To evaluate the in-vitro free radical scavenging activity of leaves Mentha pulegium linn. Petroleum ether, ethanol, aqueous extracts of Mentha pulegium were prepared, with successive extraction in soxhlet apparatus. Each extract was selected to study the free radical scavenging activity by superoxide scavenging assay method. It was found that aqueous extract contained carbohydrates, glycosides amino acids flavonoids, tannins, alkaloids, steroids; ethanolic extract contained glycosides amino acids flavonoids, tannins, alkaloids, steroids. Ethanolic extract of Mentha pulegium has showed 58.70.62 % inhibition in superoxide scavenging model. Aqueous extract also showed almost similar activity (54.90.53 % compared to ethanolic extract), while Petroleum ether extract showed poor inhibition of superoxide scavenging activity. All extracts showed dose and time dependent inhibition of superoxide scavenging activity. Jatropha gossypifolia had the highest total phenolic content (42.60 mg TAE (tannic acid equivalent)/ 100 g fresh weight). Total phenolic content had positive correlation with antioxidant capacity. This shows that the plants, especially Mentha pulegium, may be potent source of natural antioxidants.

Keywords: Mentha pulagium, DPPH, Phenolic content, Superoxide scavenging, Antioxidant activity.


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