Abstract

PHYTOCHEMICAL SCREENING OF CURCUMA CAULINA J. GRAHM RHIZOME EXTRACT BY USING HRLC-MS TECHNIQUE

Anand S. Jadhao* and Anil S. Bhuktar

ABSTRACT

The aim of present study was to characterize the presence of biologically active phytochemical constituents of Curcuma caulina J. Grahm methanolic rhizome extracts by using High Resolution Liquid Chromatography - Mass Spectroscopy (HRLC-MS) technique. The mass spectra of compounds found in the extract was matched with Metlin database library, results confirmed the presence of therapeutically potent chemical compounds in C. caulina rhizome extract. The metabolites analysis by ESI-Q-TOF-MS revealed the presence of 14 major abundant compounds namely Phe Tyr Lys, 1alpha,25-dihydroxy-26,27-dimethyl-20,21,22,22,23,23-hexadehydro- 24a- homovitamin D3 /1alpha,25- dihy, Tolperisone, Ala Ile Ile, Punctaporin B, beta-vinyl acrylic acid, Rotoveratrine A, (25R)-26,26,26-trifluoro-1alpha,25- dihydroxyvitaminD3/(25R)-26,26,26 trifluoro-1alpha,25-dihydrox, alpha- Aminodiphenylacetic acid, Madecassic acid, Levonorgestrel oxime, 1,25-Dihydroxyvitamin D3 3- glycoside, Neoxanthin and Pancuronium.

Keywords: Phytochemical, HRLC- MS, Curcuma caulina.