SPECTROPHOTOMETRIC ESTIMATION OF CHLORTHALIDONE IN PHARMACEUTICAL FORMULATION USING MBTH & FC REAGENT
H.Padmalatha*, V Anupama, B Parijatha, K Rama
ABSTRACT
Two visible spectrophotometric methods have been developed for the
determination of Chlorthalidone either in pure form or in their
pharmaceutical formulations. The developed methods are based on
reaction of Chlorthalidone with MBTH, FC Reagent. They are
quantified spectrophotometrically at their absorption maximum at
540nm (Method A), 680nm (Method B). Beer’s law was obeyed in the
concentration ranges of 10-50 μg/ml, 10-50 μg/ml and 10-50 μg/ml for
the two methods respectively. The colors were found to be stable for
more than 4 hours. The proposed methods were successfully applied
for determination of the Chlorthalidone in their pharmaceutical
formulations and the results compared favorably to that of reference
methods, hence are recommended for quality control and routine
analysis.
Keywords: Spectrophotometer, Pharmaceutical dosage forms, Chlorthalidone, MBTH, FC Reagent.
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