Abstract

EVALUATION OF CEFIXIME ANTIBIOTIC RESISTANCE: RELATION OF ANTIBIOTIC QUALITY PARAMETERS TO CASES OF RESISTANCE OCCURRING IN ARIS DISEASE IN TASIKMALAYA CITY HEALTH CENTER

Danni Ramdhani* and Sri Agung Fitri Kusuma

ABSTRACT

Objective. Cefixime is an antibiotic that usually used in treatment of acute respiratory infections (ARIs). One of the factors that support the occurrence of cases of antibiotic resistance is the antibiotic quality parameters of the potential and levels of antibiotics used. This study aims to determine the relationship between quality parameters of cefexime antibiotics used in public health centers in Tasikmalaya city with cases of cefexime resistance occurring in the area. Methods. This research method includes the determination of antibiotics potential with 3+3 pattern and the comparative value of the activity using agar diffusion method perforation techniques and antibiotic assay with UVVisible spectrophotometry method. Results. The research shows the antibiotic potential of cefixime is 95.86%. The validation method demonstrate that the method used fulfill the requirements of standard curve with concentrations in the range of 6-14 mg/L with a correlation coefficient of 0.995. The method used demonstrated accuracy with an average recovery percent of 100.08 ± 2.39% and a precision of 0.06%. LOD value of the method used is 0.1236 mg/L while the LOQ value is 0.4121 mg/L. The Results of analysis showed levels of cefixime is 100.71%.

Keywords: ARIs, antibiotics, cefixime, potency, antibiotic assay, validation method.