Abstract

ASCERTAINING DRUG RESISTANCE BACTERIA FROM HOSPITAL DRAINAGE WATER AND EVALAUTING ITS SUSCEPTIBILITY WITH LEAF EXTRACT OF BARLERIA PRIONITIS

K. Padmalochana* and R. Saranya

ABSTRACT

To study the identification of drug resistant bacteria from hospital sewage water especially from drainage canal. The sample was processed for enumerating the total bacterial count and showed a total population of 2.3×104. The BOD level of the sample as it is a liquid was evaluated using titration method. The major part of the work denoted by isolating the drug resistant bacteria by cultivating the bacteria in media containing different class of drug. The antibiotic is applied in the drug resistant bacteria were Vancomycin, Amoxicillin, Cephalexin, Amikacin. The identified bacteria S. aureus, Klebsiella species, Pseudomonas species was determined for its multidrug resistant property by antibiogram using different generation antibiotic and zone integration. The antibiogram of the isolated bacteria expression of antibiotic resistant characters by producing beta lactamase (penicillin, cephalexi), 6’-N. acetyl transferases (Amikacin), D-alanine TD-alanine transpeptidase (vancomycin), OXA-1 beta- lactamase (amoxicillin). The enzyme activity was determined by nitrocefin test. The drug resistant bacteria is treat with Barleria prionitis methanolic leaf extract which yield 13.93% of the phytoconstituent by qualitatively. The B.prionitis was resistant to the drug resistant bacteria.

Keywords: Drug resistant bacteria, Barleria prionitis, Antibiotics, Enzymes.