Abstract

EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF EXTRACTS OF BALANITES AEGYPTIACA FRUITS IN EXPERIMENTALLY INDUCED HEPATIC DAMAGE IN RATS

*Supriya C. S. and Shivalinge Gowda K. P.

ABSTRACT

Alcoholic (ABA) and aqueous extracts (AQBA) of the Balanites aegyptiaca fruits were used for the study of hepatoprotective activity against carbon tetrachloride (CCl4) .The doses were determined from the acute oral toxicity study in albino mice. The extracts were administered orally (500 and 200mg/kg) for 10 days in CCl4 model respectively. The CCl4 (10 days) was administered to all the groups except normal vehicle treated rat. Silymarin (100mg/kg) was used as a standard hepatoprotective drug. After 24 h of the last treatment, blood was withdrawn by retro orbital puncture and serum SGPT, SGOT, ALP, bilirubin were estimated. The SOD, GSH and LPO levels were estimated from the liver PMS and histopathology examination was carried out from the portion of the liver tissue. The result indicated preliminary phytochemical investigation confirms the presence of flavonoids, saponins, phytosterols, fixed, fats, alkaloids, glycosides in AQBA and ABA. The results indicated that serum SGPT, SGOT, ALP, bilirubin, liver tissue LPO levels significantly increased and liver tissue SOD, GSH levels decreased significantly in CCl4 induced hepatotoxic rats whereas these values significantly altered towards the normal values in AQBA, ABA, and Silymarin treated rats when the results are compared to normal and toxic control group. The histopathology study reveals the protective effect of AQBA, ABA, and Silymarin.

Keywords: Serum glutamate oxaloacetate transaminase, Carbon tetra chloride, Silymarin.