Abstract

ANTIBACTERIAL ACTIVITY OF CHENOPODIUM ALBUM EXTRACT

Dr. Kalpana Teware*

ABSTRACT

For the studies of Antibacterial effect of phytochemicals obtained from Chenopodium album extract a medicinally important plant, microbial successfully procured from Microbial Culture collection, National Centre for Cell Science, Pune, Maharashtra, India The lyophilized cultures of bacterial strains upon culturing in nutrient broth for 24-48 hours at 37oC in an incubator resulted into turbid suspension of activated live bacterial cell ready to be used for microbiological study. The lawn cultures were prepare with all the microbes used under present study and sensitivity of bacteria towards the extracts obtained from the Chenopodium album was studied at the concentration of 100mg/ml using well diffusion method. The present investigation evaluated against bacterial pathogens. The fresh pure 100% extracts obtained from plant used to suitably dilute up to the concentrations of 100, 50 and 25 mg per ml and applied using cup plate agar diffusion method against S. Mutans and Proteus mirabilis was compared with ciprofloxacin standard. Antibacterial activity data revealed that Chenopodium album leaf showed promising antibacterial activity especially towards Gram negative and Gram positive bacteria. well diffusion method. Results of the experiment clearly show the antibacterial activity of extracts of Chenopodium album. This is very promising because it is reported that plant extracts are more active against Gram negative and Gram positive bacteria and the search is always going on for plants extracts which are able to inhibit the dangerous Gram negative bacteria. Studies are needed to isolate and characterize the bioactive principles to develop new antimicrobial drugs.

Keywords: antibacterial, S. mutens, Proteus mirabilis, Plant extract.