Abstract

IN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY STUDIES ON LEAF EXTRACT OF GREWIA HETEROTRICHA MAST AND GREWIA SERRULATA DC.

Usha B. and Pushpalatha K. C.*

ABSTRACT

Methanol leaf extract of Grewia heterotricha Mast. and Grewia serrulata DC. (Family: Malvaceae) were assessed for their antioxidant and anti-inflammatory activity by invitro methods. Quantitative analysis of phytocomponents such as total phenolics, flavonoids and tannins were estimated by standard spectrophotometric methods. The results obtained showed that the methanol leaf extract of G.heterotricha (MEGH) and G.serrulata (MEGS) have significant DPPH radical scavenging and H2O2 scavenging activity with IC50 values 98.95μg/ml, 110.1μg/ml (MEGH) and 102.1μg/ml, 143.3μg/ml (MEGS) respectively. Extracts also exhibited good reducing ability of 131.8 ±11.67 mg ascorbic acid/g dry extract (MEGH) and 79.92 ±5.92 mg ascorbic acid/g dry extract (MEGS). Methanol extracts of both the plants showed promising anti-inflammatory activity by inhibiting protein denaturation with IC50 values 1440μg/ml (MEGH) and 1685μg/ml (MEGS). HRBC membrane stabilizing assay of MEGH and MEGS showed significant inhibition values of 91.33±0.25% and 88.11±0.24% respectively. Proteinase inhibitory activity of MEGH and MEGS was found to be 67.91±0.08% and 52.81±0.12% respectively. The results obtained in the present study suggest that the phytocomponents like phenolics, flavonoids and tannins present in the extracts may be responsible for antioxidant and anti-inflammatory activity and hence can be a potent source for the development of therapeutic drugs.

Keywords: Anti-inflammatory, protein denaruration, proteinase inhibition, HRBC.