Abstract

ANTIOXIDANT SCREENING AND POLYPHENOLS PROFILING OF IPOMOEA ASARIFOLIA (DESR.) ROEM. & SCHULT (CONVOLVULACEAE)

MEDA Nâg-Tiero Roland*, COMPAORE Moussa, BANGOU Mindiédiba Jean, VLASE Laurian, KIENDREBEOGO Martin, OUEDRAOGO Anicet Georges

ABSTRACT

Ipomoea asarifolia, native to tropical America is widely used in Africa traditional medicine for the treatment of various diseases. This plant is well used in Burkina Faso folk medicine to manage malaria, convulsions and rheumatism. For that, the organic fractions of I. asarifolia hydroacetonic extract have been screened for their antioxidant activity and polyphenols profile. The ferric reducing antioxidant power (FRAP), the ABTS radical cation scavenging capacity and the free radical scavenging capacity (DPPH) were used to evaluate the antioxidant abilities. The total phenolics and flavonoids were quantified using Folin-ciocalteu and aluminium chloride tests, respectively and the phenolic compounds were analyzed by HPLCMS. The results obtained have demonstrated that the ethyl acetate fraction exhibits a remarkable antioxidant according to the three methods. In this study gentisic, p-coumaric and ferulic acids and hyperoside, isoquercitrin, quercitrin and quercetol were identified. Reported chlorogenic and caffeic acids were also found. These finding demonstrated that I. asarifolia extracts contained some antioxidant compounds which could support the traditional uses.

Keywords: Polyphenols; Antioxidant activity; Ipomoea asarifolia; HPLC-MS.