Photo Gallery

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from  7.454 to 7.632  due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • WJPPS FEBRUARY ISSUE PUBLISHED
  • FEBRUARY 2021 Issue has been successfully launched on 1 February 2021.

Abstract

THE EFFIECIENT METHOD FOR DNA EXTRACTION FROM BLOOD SPOT ON JEANS

Rasha Sadeq Ameen*

ABSTRACT

When a crime or other incident takes place, clothing items are one of the most common types of evidence that present or left behind in the crime scenes, and can become directly involved in the case itself. Blood sample was collected from (5) voluntaries and spotted on three different color of blue jeans at different time (directly, after 2 and 4 weeks), the DNA was extracted by using the most commonly method (Chelex® 100, organic phenol-chlorophorm, Wizard® Genomic DNA Purification Kit (Promega, USA). Results indicated that the organic phenol- chlorophorm method cannot be used for DNA extraction from the dark blue type of the jeans while there is decrease in DNA concentration with increase in time of spot dry in both chelex and kit methods, in the case of the medium blue type of the jeans the organic
method gave the best result with mean DNA concentration (4.306 ng/μl) from direct treatment and (3.145 ng/μl) after 2 week of drying, also the phenol- chloroform organic method is the best method for the extraction of DNA from light blue jeans directly with 12.162 ng/μl mean concentration of DNA followed by the kit method with 1.19 ng/μl mean concentration of DNA, these value was become 3.848 ng/μl and 0.0194 ng/μl respectively after 2 week of extraction. After 4 week of drying the spot there is slightly difference in the amount of the mean concentration of DNA obtained from chelex and kit method while there is no DNA obtained when using the organic method for extraction. From that it has been concluded that there is a correlation between the obtained DNA concentration and the color of the jeans in which the blood spot left on.

Keywords: bloodspots, phenol-chlorophorm organic, chelex® 100, DNA extraction, jeans.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More