Abstract

EVALUATION OF VARIOUS METHODS FOR DETECTION OF BIOFILM FORMATION AMONG THE MULTIDRUG-RESISTANT UROPATHOGENIC ESCHERICHIA COLI ISOLATES

Pravin Kumar Chaudhary, Sunil Shekhar Ghimire* and Varsha A. Singh

ABSTRACT

Biofilm is a well-organized, cooperating community of microorganisms. A Total of 60 urine isolates of Escherichia coli strains resistance to cephalosporins and floroquinolones were subjected to biofilm detection methods i.e. Tissue Culture plate (TCP) method, Tube method (TM), Congo Red Agar method and Modified Congo Red Agar method (MCRA).Which help to find the efficacy of mentioned tests method for detection of biofilm formation by Uropathogenic Escherichia coli. The isolation Rate of uropathogenic Escherichia coli was 70.34% and Multidrug-resistant (strains resistant to cephalosporins and floroquinlones) were58.82%. Among them 61.67% Multidrug-resistance Escherichia coli were biofilm producers. Rate of biofilm production in tube adherance method, modified congored agar method and tissue culture plate method in combination was 61.68% and decreased as per number of tests.In TCP strains showed O D values as weak (51.66%), high (28.33%) and followed by moderate (20%). So the Phenotypic methods i.e. Tissue Culture plate (TCP) method, Tube method (TM), Congo Red Agar method, Modified Congo Red Agar method (MCRA) are relatively better tool for assay.

Keywords: Uropathogenic Escherichia coli, cephalosporins and floroquinolones.