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G. Shobana*, Dr. N. Agnel Arul John and S. Jasmin Vinitha


Inflammation is a pathophysiological response of living vascularised tissue to an injury. The natural products today symbolize safety in contrast to the synthetic drugs that are regarded as unsafe to human and environment. Number of herbal medicines are recommended for the treatment of inflammation that has no side effects. The present study was aimed to evaluate the anti inflammatory activity of poly herbal formulation(PHF) on freund’s adjuvant induced paw edema in albino rats. A poly herbal formulation was prepared by using three common plants like Calendulo officinalis L. (Asteraceae family), Lantana camara L. (Verbanaceae family), and Desmodium gangeticum Linn. (Fabaceae family). In present study, wistar strains of albino rats were used and the animals were divided into six groups and treated accordingly: Normal control, Freund’s adjuvant treated group (0.1ml/kg bw in sub plantar region), Freund’s adjuvant + standard drug indomethacin (10mg/bw orally), Freund’s adjuvant + PHF (100, 200 and 300mg/kg bw). After the experimental period, the tissue samples were collected for analysing various biochemical parameters. The administration of freund’s adjuvant to the experimental animals produced reduction in the levels of SOD, GSH, GPX and catalase. A significant increase in the length of paw thickness, SGOT, SGPT and lipid peroxide, hydroxyl proline and hexosamine, hydroxyl proline was noted in the animals induced with freund’s adjuvant. The animls treated with poly herbal formulation (PHF) at dose level of 100,200 and 300kg/bw significantly normalise the above parameters. Administartion of standard drug indomethacin also showed similar results compared to PHF treated animals. The results conclude that the poly herbal formaulation has a potent anti inflammatory effect against freund’s adjuvant induced paw edema in albino rats.

Keywords: Inflammation, Hydroxyproline, Hexosamine, Lantana camara L. Indomethacin, etc.

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