Abstract

MIRACLE ENZYMES SERRAPEPTASE AND NATTOKINASE MITIGATE NEUROINFLAMMATION AND APOPTOSIS ASSOCIATED WITH ALZHEIMER’S DISEASE IN EXPERIMENTAL MODEL

*Hanaa H.Ahmed, Nevein, N. Fadl, Karima, A. El- Shamy, Amal H. Hamza

ABSTRACT

Alzheimer’s disease (AD) is a challenging neurodegenerative disorder in the elderly that is characterized by impairment of memory and eventually by disturbances in reasoning, planning, language and perception. The current study was planned to elucidate the protective role of the proteolytic enzymes, serrapeptase and nattokinase, in ameliorating neuroinflammation and apoptosis associated with AD induced in rats. Sixty adult male albino rats were enrolled in the present study and randomly classified into six groups; Group ( 1) set as control group, Group (2) AD induced group in which rats were orally administered with AlCl3 (17 mg/ kg b.wt. ) for 45 days and Groups from (3) to (6) were orally administered with AlCl3 for 45 days and simultaneously supplemented with low and high doses of serrapeptase ( 10.800 U/ kg b.wt. and 21.600 U/ kg b.wt.) and nattokinase (360 FU/ kg b.wt. and 720 FU/ kg b.wt.) respectively. Brain cholinesterase activity, TGF-β , IL-6 , Bcl- 2 and P53 levels were estimated as well as histological investigation of the brain tissue of the different studied groups was carried out. In comparison with the control group, AlCl3 administration produced significant elevation in cholinesterase activity, TGF- β ,IL-6 and P 53 levels while it induced significant reduction in Bcl-2 level . Regarding the groups treated with either serrapeptase or nattokinase in different doses, our findings showed significant decrease in cholinesterase activity, TGF- β ,IL-6 and P53 levels accompanied with significant increase in Bcl-2 level as compared to untreated AD induced group. These results are greatly supported by the histological findings. Thus, it could be concluded that serrapeptase and nattokinase may be considered as newly neuroprotective agents against inflammation and apoptosis characterizing AD through their proteolytic, anti-inflammatory and anti-apoptotic effects.

Keywords: Alzheimer’s disease, Proteolytic enzymes, Inflammation, , Apoptosis, Rats.