MIRACLE ENZYMES SERRAPEPTASE AND NATTOKINASE MITIGATE NEUROINFLAMMATION AND APOPTOSIS ASSOCIATED WITH ALZHEIMER’S DISEASE IN EXPERIMENTAL MODEL
*Hanaa H.Ahmed, Nevein, N. Fadl, Karima, A. El- Shamy, Amal H. Hamza
ABSTRACT
Alzheimer’s disease (AD) is a challenging neurodegenerative disorder
in the elderly that is characterized by impairment of memory and
eventually by disturbances in reasoning, planning, language and
perception. The current study was planned to elucidate the protective
role of the proteolytic enzymes, serrapeptase and nattokinase, in
ameliorating neuroinflammation and apoptosis associated with AD
induced in rats. Sixty adult male albino rats were enrolled in the
present study and randomly classified into six groups; Group ( 1) set as
control group, Group (2) AD induced group in which rats were orally
administered with AlCl3 (17 mg/ kg b.wt. ) for 45 days and Groups
from (3) to (6) were orally administered with AlCl3 for 45 days and
simultaneously supplemented with low and high doses of serrapeptase
( 10.800 U/ kg b.wt. and 21.600 U/ kg b.wt.) and nattokinase (360 FU/
kg b.wt. and 720 FU/ kg b.wt.) respectively. Brain cholinesterase activity, TGF-β , IL-6 , Bcl-
2 and P53 levels were estimated as well as histological investigation of the brain tissue of the
different studied groups was carried out. In comparison with the control group, AlCl3
administration produced significant elevation in cholinesterase activity, TGF- β ,IL-6 and P
53 levels while it induced significant reduction in Bcl-2 level . Regarding the groups treated
with either serrapeptase or nattokinase in different doses, our findings showed significant decrease in cholinesterase activity, TGF- β ,IL-6 and P53 levels accompanied with significant
increase in Bcl-2 level as compared to untreated AD induced group. These results are greatly
supported by the histological findings. Thus, it could be concluded that serrapeptase and
nattokinase may be considered as newly neuroprotective agents against inflammation and
apoptosis characterizing AD through their proteolytic, anti-inflammatory and anti-apoptotic
effects.
Keywords: Alzheimer’s disease, Proteolytic enzymes, Inflammation, , Apoptosis, Rats.
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