Abstract

NEPHRO-PROTECTIVE AND HEPATO-PROTECTIVE PROPERTY OF COMMELINA DIFFUSA LEAF EXTRACT IN DOXORUBICIN-INDUCED ALBINO RATS.

Sule O. J.*, Arhoghro E. M. and Erigbali P.

ABSTRACT

Ethanol leaf extract of commelina diffusa at 200 and 400mg/kg was administered to albino rats for 20 days and simultaneously treated with doxorubicin (cumulative dose of 15 mg/kg i.p. in six divided doses 2.5mg/kg body weight i.p of doxorubicin on 7th, 10th,13th, 16th and 19th day of study). On the 20th day, AST (Alanine amino transferase), ALT (Aspartate amino transferase), TB (total bilirubin), Urea and Creatinine were evaluated, using the various standard kits. Pretreatment with leaf extract of Commelina diffusa at 200 and 400 mg/kg showed a significant decrease in the levels of AST, ALT, TB, Urea and Creatinine when compared with the positive control (doxorubicin treated group) p< 0.05. The kidney sections obtained from doxorubicin treated animals showed, necrotic tubules with marked perforation and intense distortion of tissue architecture. Animals pretreated with extract of Commelina diffusa at 200 and 400 mg/kg showed normal kidney stroma with Bowman’s capsule with glomerulus and renal tubules. Also, the liver sections obtained from doxorubicin treated animals showed infiltration of neutrophils with spotty inflammation. Animals pretreated with extract of Commelina diffusa at 200 and 400 mg/kg showed improvement in the cell integrity evidenced by normal hepatic stroma with hepatocytes, sinusoid and central vein. The results obtained from this study show that the ethanol leaf extract of Commelina diffusa may possess nephron-protective and hepato-protective functions against doxorubicin-induced wistar albino rats.

Keywords: Commelina diffusa, Doxorubicin, Alanine amino transferase, Aspartate amino transferase, nephron-protective, hepatoprotective.