Abstract

ANTIBACTERIAL ACTIVITIES OF IRAQI PROPOLIS AND ITS ACTIVE COMPONENTS EXTRACTS ON SOME BACTERIAL ISOLATES (IN VITRO STUDY)

*Shaimaa Obead Hasson

ABSTRACT

Background: Propolis (bee glue) is a resinous product that honeybees collect from living plants and use in construction and adaptation of their hives. It is extensively used in folk medicine, and a number of investigations have shown that propolis posses antibacterial, antiviral and antifungal properties. Aims: In this paper evaluated the antibacterial activity of propolis and its active component against ten bacterial pathogens. Methods :The antibacterial effects of propolis and active component of propolis against 5 Gram-positive and 5 Gramnegative bacterial isolates, including Staphylococcus aureus marsa, Staphylococcus epidermidis, Streptococcus mutanus, Enterococcus feacalis, Streptococcus pneumoniae, , Pseudomonas fluresence, Escherchia coli, Proteus mirabilis, Proteus vulgaris, and Acinetobacter, all of them were studied. Results: Antimicrobial activities of crude ethanol extract of Kerbalaa propolis (EEKP) and active components of propolis at 25% concentration against bacterial isolates were studied. The results of agar diffusion at 25% concentration showed that most bacterial isolates were sensitive to EEKP. S. pneumoniae was highly sensitive to EEKP than other Gram positive and Gram negative bacteria with inhibition zones was 30mm. all bacterial isolates were highly sensitive to active component of propolis. Showing the maximum zone of inhibition of 30 mm at the 25% concentration Conclusions: In summary, the propolis and its active component extract showed a wide spectrum activity and appears to satisfy all of the criteria for antibacterial agents. These results suggest that propolis can be used to protect food and reduce the risk of contamination from pathogenic microorganisms.

Keywords: propolis, active component of propolis, and antibacterial activity.