SPECTROPHOTMETRIC DETERMINATION OF SOME FLOUROQUINOLONE DRUGS IN TABLETS
Gehad G. Mohamed, Shaban M. Khalil, Samah H. Mahmoud
ABSTRACT
This work described the use of spectrophotometric methods for the
determination of some antibiotic flouroquinolone drugs namely
sparfloxacin (SFX), levofloxacin (LFX), ofloxacin (OFX) and
enrofloxacin (EFX) in pure form and in tablets. The first method is
based on charge transfer complex formation between these drugs as nelectron
donors and 7,7,8,8-tetracyanoquinodimethane (TCNQ) and
5,6-dicyano-1,4-benzoquinone (DDQ) as π-acceptors. The
experimental conditions for these CT reactions have been carefully
optimized. Beer’s law is valid over the concentration range from 5-150
and 5-180 μg mL-1 of SFX, LFX and OFX and SFX, LFX and EFX
drugs using DDQ and TCNQ reagents, respectively. The second
method is based on ion-pair formation between these drugs and
Mo(V)-thiocyanate reagent in sulphuric acid medium and extraction
with 1,2-dichloro-ethane. Beer’s law is valid over the concentration
range from 10-150, 10-125, 10-90 and 10-90 μg mL-1 for SFX, LFX, OFX and EFX drugs,
respectively. Different analytical parameters namely molar absorptivity (), standard
deviation, relative standard deviation, correlation coefficient, limit of detection and
quantification are calculated. The results obtained by the proposed methods are in good
agreement with those obtained by the official method as indicated by the percent recovery
values. The methods are applied for the determination of these flouroquinolone drugs in
tablets.
Keywords: Floroquinolone drugs; DDQ; TCNQ; Mo(V)-thiocyanate; spectrophotometry; charge-transfer; ion-pair formation.
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