Abstract

IN-VITRO ANTICANCER ACTIVITY EVALUATION OF WITHANIA COAGULANS ON BREAST CANCER CELL LINES

Gopinath S. M., Ismail Shareef M.*, Sonia Gupta and Ayushi Gupta

ABSTRACT

Breast cancer has become issue of major concern because of its increasing prevalence worldwide. Synthetic chemotherapeutic agents fail to treat it as tumor cells tend to develop resistance against the Plant derived chemotherapeutic agents offers a high potential to combat the situation. Though much research is done on this field, but still many plants are yet to be explored for their anti carcinogenic properties against breast cancer. In the present study we analyzed the ethanol extract of Withania coagulans for its cytotoxic and hemolytic properties. Cell cycle studies shows that MCF7 cells when subjected to 320μg/ml of plant extract was able to arrest 25.99% of MCF7 cells at G2M phase. Also, the plant extract is non hemolytic and thus, can be prove to of high potential against breast cancer.

Keywords: Adenocarcinoma, withnaolides, caspase cascade, FACS.