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Abstract

PREVALENCE OF BLA CTX-M EXTENDED SPECTRUM BETA LACTAMASE GENE IN UROPATHOGENIC ESCHERICHIA COLI

*N. Vidhya, S.S Sudha

ABSTRACT

Purpose: Extended spectrum beta lactamases (ESBLs) have been observed in virtually all the species of family Enterobacteriaceae. The enzymes are predominantly plasmid mediated and are derived from broad-spectrum beta lactamase by a limited number of mutations. The aim of this study was to determine the prevalence of the bla CTX-M gene in Uropathogenic Escherichia coli isolates obtained from tertiary care hospitals in and around Coimbatore, South India. Methods: Ten Uropathogenic E. coli (UPEC) isolates were analyzed regarding the presence of the bla CTX-M gene, using PCR which were initially screened by phenotypic methods. CTX-M gene was detected in only 8 UPEC isolates from tertiary care hospitals. Results: The study concludes that there was a high prevalence of the bla CTX-M gene (80 %) among ESBL positive E. coli isolates from the UTI patients using CTX-M primer.

Keywords: ESBL, Urinary tract Infections, E.coli, CTX-M, Multidrug resistance.


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