PREVALENCE OF BLA CTX-M EXTENDED SPECTRUM BETA LACTAMASE GENE IN UROPATHOGENIC ESCHERICHIA COLI
*N. Vidhya, S.S Sudha
ABSTRACT
Purpose: Extended spectrum beta lactamases (ESBLs) have been
observed in virtually all the species of family Enterobacteriaceae. The
enzymes are predominantly plasmid mediated and are derived from
broad-spectrum beta lactamase by a limited number of mutations. The
aim of this study was to determine the prevalence of the bla CTX-M
gene in Uropathogenic Escherichia coli isolates obtained from tertiary
care hospitals in and around Coimbatore, South India. Methods: Ten
Uropathogenic E. coli (UPEC) isolates were analyzed regarding the
presence of the bla CTX-M gene, using PCR which were initially
screened by phenotypic methods. CTX-M gene was detected in only 8
UPEC isolates from tertiary care hospitals. Results: The study
concludes that there was a high prevalence of the bla CTX-M gene (80
%) among ESBL positive E. coli isolates from the UTI patients using
CTX-M primer.
Keywords: ESBL, Urinary tract Infections, E.coli, CTX-M, Multidrug resistance.
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