Photo Gallery

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from  7.454 to 7.632  due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • WJPPS APRIL ISSUE PUBLISHED
  • APRIL 2021 Issue has been successfully launched on 1 April 2021.

Abstract

ANTIVIRAL AND ANTIBACTERIAL SUSCEPTIBILITY OF ACETONE LEAF EXTRACT OF COMBRETUM PANICULATUM

Nwobodo H. A.*

ABSTRACT

Leaf extract of Combretum paniculatum is used among some local folks in Nigeria for treating viral and bacterial infections. To understand the scientific basis for such practice, the antimicrobial activity of acetone leaf extract of Combretum paniculatum was investigated against selected viruses (Peste des petits ruminant virus (PPRV), Infectious bursal disease virus (IBDV), New castle disease virus (NDV)) and bacteria (Escherichia coli, Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, Salmonella Typhimurium and Clostridium perfringens) using tissue culture, embryonated chicken eggs and agar well diffusion test respectively. The phytochemical analysis of the leaf was also performed using standard methods. Result showed that the leaf extract exhibited no activity against the viruses at concentrations less than or equal to 10-3.3, 10-3.6 and 10-3.6 mg/ml which were the highest concentrations not toxic to Vero cells, Chicken embryo fibroblast cells and 11 day old chicken embryo respectively, but inhibited the growth of Salmonella typhimurium and Bacillus cereus in a concentration dependent manner. At 50 mg/ml, 7 mm and 5 mm diameters of zone of inhibition were recorded against Salmonella typhimurium and Bacillus cereus respectively. With increase in concentration of the extract up to 400 mg/ml, there was a corresponding increase in zones of inhibition (33 mm and 24mm against Salmonella typhimurium and Bacillus cereus respectively). Higher minimum inhibitory concentrations (MICs) of 6.25 mg/ml and 12.5 mg/ml and minimum bactericidal concentrations (MBCs) of 12.5 mg/ml and 25.0 mg/ml against Salmonella Typhimurium and Bacillus cereus was observed compared with 0.30 mg/ml of the control (L-flox). Phytochemical analysis showed the presence of alkaloids (+ + +), tannins (+ + +), flavonoids (+), lipids (+), proteins (+ + +), reducing sugar (+ +), and saponins (+ + +), indicating the possible presence of bioactive agent capable of inhibiting the growth of Salmonella typhimurium and Bacillus cereus. Purification of the crude extract is necessary to determine the bioactive substances responsible for the observed antibacterial effect.

Keywords: Antimicrobial, Susceptibility, Phytochemistry, Combretum Paniculatum.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More