Abstract

GREEN SYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES FROM BRYUM SALAKENSE CARD. A MOSS PLANT (BRYOPHYTE)

A. Vimala* and S. Sahaya Sathish

ABSTRACT

Objectives: Metal nanoparticle synthesis is fastest growing area of research in life science. Nanotechnology applied to reduce the material into nano size. Plant mediated synthesis of nanoparticle is ecofriendly compare to other methods of synthesis. Present investigation aims to produce silver nanoparticles by green synthesis using aqueous extract of Bryum salakense. Methods: The plant extract mixed with 1mM of AgNo3 kept in light condition. The synthesized AgNps were characterized by UV-Vis Spectroscopy, FTIR, FESEM, EDAX, Zeta potential and XRD. Both plant extract and silver nanoparticles were tested against the human bacterial pathogens viz. Staphylococcus lentus and Staphylococcus aureus (gram positive) Escherichia coli, Klebsiella pneumoniae and Serratia marcescens (gram negative). Result: Colour changes during the incubation of extract with AgNo3 from pale yellow to reddish brown indicating the silver nanoparticle formation. λ max at 443 nm in UV-Vis confirms the presence of AgNps. FTIR analysis revealed the various functional groups were acted as capping agent for the production of silver nanoparticles. FESEM and XRD analysis revealed the average size of the synthesized particles were between the 35nm and 40nm. Zeta potential for synthesized AgNps was found to be -33mv. It confirmed the synthesized AgNps have a high stability. Compare to plant extract, the silver nanoparticles showed a higher inhibition rate in all the tested bacteria. Conclusion: it is concluded that Bryum salakense could be a good source for synthesis the silver nanoparticles and also acted as good antimicrobial agent.

Keywords: Bryum salakense, silver nanoparticles, UV-Vis, FTIR, FESEM, XRD and antibacterial activity.