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Pankaj Kumar Patel*, Dr. Anup Kumar, Sandhyavali M.S., Priyadarshini S.R., Dr. Ambrish Kumar Singh


Microbial reduction of ketones is gaining importance in synthetic chemistry as an alternative tool for the synthesis of enantiomeric alcohols. To date biocatalytic reduction of Acetophenone and its derivatives has been widely reported. We have made an attempt to screen some of the microorganisms which were obtained from culture collection centre as well as those isolated in our microbiology lab, for their potential and performance in reduction of ketones. Among the screened microorganisms, used for the reduction of Acetophenone and its derivatives, Aspergillus flavus, Aspergillus niger showed significant bioconversion where as Candida viswanathii and Sacchromyces crevisae exhibited the least conversion. As Aspergillus flavus showed maximum ability of bioreduction, it was considered for optimization. The parameter evaluated during optimization includes pH, temperature, and substrate concentration and incubation time. The optimization conditions at which maximum bioconversion was achieved where pH 7.6, temperature 300C, incubation time 48hours and substrate concentration 0.2ml (238.2mg).

Keywords: Acetophenone, p-chloroacetophenone, Aspergillus flavus, Aspergillus niger, Candida viswanathii, bioreduction, optimization.

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