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Abstract

A UV SPECTROPHOTOMETRIC ASSAY METHOD FOR AVAILABLE BRANDS OF ONDANSETRON HYDROCHLORIDE

V. M. Biju*, S. Naga Gayatri and Alen Sam Thomas

ABSTRACT

A simple, reliable, rapid and a novel method have been developed for determination of ondansetron hydrochloride. The method is truly based on measuring UV absorbance maximum of 210 nm using universal solvent i.e. double distilled water (DDW). Three different brands of ondansetron were purchased from market for the analysis. A standard solution of drug was initially prepared by dissolving 10 mg of standard drug in double distilled water to get concentration of 100μg/ml. The different aliquots of solution of three different brands were prepared in the range of 2- 10 μg/ml. The percentage assay of each brand was calculated and their regression analysis was also performed. Among the three brands (Emeset, Zofer and ondem) Emeset showed high percentage assay value of 100.77 and remaining two brands showed same percentage assay value of 97.22. All the brands were found to be within the limit as per Indian pharmacopeia. The regression coefficient was with in tolerable limit i.e. 0.999.

Keywords: Ondansetron Hydrochloride, UV Spectrophotometry, Emeset, Zofer, Ondem, percentage assay.


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