Abstract

DESIGN AND DEVELOPMENT OF OPHTHALMIC NANOEMULSION FORMULATION FOR REDUCING OCULAR HYPERTENSION

Reshu Gupta*, Tejal G. Soni and B.N. Suhagia

ABSTRACT

The objective of present study was to design and develop an ophthalmic nanoemulsion formulation for reducing ocular hypertension. Medium Chain Triglyceride was selected as oil phase due to the high solubility of Brinzolamide in it as compared to other oils and Polyoxyl 35 castor Oil and Propylene glycol were used as surfactant and cosurfactant respectively. The construction of nanoemulsion was further optimized by D-optimal mixture design, taking oil, Smix and water as independent variables and droplet size and drug entrapment as response variables. Mathematical equations and response surface plots were used to correlate the dependent and independent variables. The optimized composition of nanoemulsion was predicted by numerical optimization technique on the basis of the highest desirability value. The predicted optimized nanoemulsion which contained 5% oil, 15% Smix, and 80% water was formulated and evaluated for ophthalmic application. The optimized nanoemulsion formulation showed droplet size (103.03±1.6nm) and drug entrapment in formulation (101.51±0.81% ) which was in close agreement with the predicted value of response variable by the optimization software, i.e. 102.983 nm and 100.038%, respectively. These results confirmed that the D-optimal mixture design can be successfully employed for designing and development of nanoemulsion based formulation of Brinzolamide.

Keywords: Nanoemulsion, Brinzolamide, Pseudoternary Phase diagram, Ophthalmic, D-optimal design.