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Abstract

DEVELOPMENT OF HPLC METHOD FOR THE DETERMINATIONOF ZINC CARNOSINE

Aakash Sahu*, Abha Doshi and Bhosale Suryakant D.

ABSTRACT

A reverse phase HPLC method is developed for the determination of zinc carnosine in pharmaceutical dosage forms. Chromatography was carried out on a C18 column [250mm, 4.6m, 5μm] using a mixture of potassium di-hydrogen ortho phosphate buffer and Acetonitrile (76:24 v/v) as the mobile phase at a flow rate of 1 mL/min. Detection was carried out at 215 nm .The retention time of the drug was 5.700 min. The method produced linear responses in the concentration range 0.5 to 60 μg/mL of zinc carnosine. The LOD and LOQ values for HPLC method were found to be 1.29 and 3.90 μg/mL respectively. The method was found to be applicable for determination of the zinc carnosine.

Keywords: HPLC, Zinc carnosine, Estimation, validation.


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