EVALUATION OF IN VITRO CYTOTOXIC ACTIVITY OF PETROLEUM ETHER, METHANOL AND AQUEOUS EXTRACTS OF INDIAN BAY LEAF, CINNAMOMUM TAMALA (BUCH.- HAM.) T. NEES & EBERM. ON CANCER CELLS
Deepavali Rajan Thanekar, Jayesh Bachu Dhodi, Archana Ramesh Juvekar*
ABSTRACT
Cinnamomum tamala (CT) (Family- Lauracea) is widely consumed
condiment and commonly known as tejpat. In present study, in vitro
cytotoxic activity of three different extracts of leaves of CT was
evaluated against human peripheral blood mononuclear cells and three
human cancer cell lines (Lung: A549, Breast: MCF-7 and Colon:
COLO 205) by Sulforhodamine B assay. Crude petroleum ether and
methanol extracts were obtained by soxhlet extraction technique and
aqueous extract by Cold maceration technique. Hemolytic activity of
extracts was also evaluated. All three crude extracts lack cytotoxic
effects on normal human cells. Whereas, different degrees of cytotoxic
activity on cancer cells by these extracts were observed. Methanol
extract; rich in phenols, flavonols and flavonoids; was found to be
significantly more active and potent against all the cancer cell lines as
compared to pet. ether and aqueous extract of CT. Lack of hemolytic
activity suggests that cytotoxic activity of CT is may be due to
apoptosis rather than membrane destabilization. The present study
suggests novel potential antitumor activity of the Indian spice CT and need for further
identification of bioactive compounds along with mechanism finding studies.
Keywords: Cinnamomum tamala, Sulforhodamine B, Tumor Cell Line, Antitumor Drug Screening Assays, Plant Extracts.
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