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Shreyansh S. Mutha, Veena S. Kasture*, Seema A. Gosavi, Rasika D Bhalke., Sarita S. Pawar


Ayurveda is the oldest surviving complete medical system in the world. Asava and Arishta are unique dosage form discovered by Ayurveda having long shelf life. Abhayarishta is an important ayurvedic formulation used to relieve the problems like piles and constipation, helpful in mild ascites and dropsy, relieve in urinary obstruction, increase digestive power and is helpful in enlargement of liver, spleen and anemia, which is prepared by fermenting the decoction of specified plant materials using fruits of Terminalia chebula. Abhayarishta a marketed ayurvedic formulation containing Gallic acid as one of the active ingredient. The objective of this study was to determine the level of alcohol, and pH in commercially available Abhayarishta to establish a routine procedure for standardization of these Ayurvedic preparations. In present communication, HPLC and UV method was developed for standardization of Abhayarishta by quantitative estimation of major compound, Gallic acid as marker as per WHO guidelines. The developed method was validated with respect to linearity, precision, accuracy, and robustness. Moreover, the alcohol content of the formulation was also determined by Gas chromatography and redox titrimetry. The formulation was also analyzed for microbial load, heavy metal content, pesticide residue, elemental analysis and pharmacological activity.

Keywords: Abhayarishta, Standardization, UV, HPLC.

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