Abstract

SCREENING AND ISOLATION OF CELLULASE PRODUCING BACTERIA FROM DUMP YARDS OF VEGETABLE WASTES

E. Venkata Naga Raju*, Goli Divakar, T. Rajesh, Akram Ghazi, Asra Pourgharashi

ABSTRACT

This work has been undertaken for the screening and isolation of cellulase producing strains of bacteria were carried out from ten soil samples, collected from dump yards of vegetable wastes various regions of Bangalore and used to screen for cellulase production by using Carboxy Methyl Cellulose (CMC) agar medium . In the present study, an attempt was made to isolate efficient cellulase producing bacteria from diverse regions of Bangalore. Different isolates were screened for possessing the ability to produce cellulase. About 6 bacterial isolates were found to be promising to produce cellulase. The organisms were tested for various biochemical tests, which leads to their identification as Bacillus licheniformis, Bacillus cereus, and Staphylococcus aureus.

Keywords: Vegetable wastes, Cellulase, Carboxy Methyl Cellulose (CMC), Bacillus licheniformis, Bacillus cereus, Staphylococcus aureus.