Abstract

A PROTOTYPE FOR BIOEMULSIFIER PRODUCTION FROM BACTERIA ITS ATTRIBUTES AS AN ANTIMICROBIAL, METAL BINDING PROSPECTS

*M. Naveen Banu

ABSTRACT

In recent years natural bioemulsifier have attracted attention because of their low toxicity, biodegradability, and ecological acceptability. Soil isolates of Pseudomonas fluorescens was used to characterize the bioemulsifier. The Emulsification Index, Emulsification activity, Drop collapse test, oil displacement method were employed for screening. Highest yield of bioemulsifier was obtained from Coconut oil. It was revealed that partially purified rhamnolipid of P. fluorescens were also screened for heavy metal resistance activity by using agar diffusion method and tube method. In this study, we investigated the synergistic antibacterial activity of rhamanolipid with Tetracycline. A rhamnolipid mixture obtained from Pseudomonas fluorescence showed inhibitory activity against Ecoli and S.aureus.

Keywords: Bioemulsifier, Antimicrobial activity, Heavy metal resistance.