WJPPS Citation

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • New Impact Factor
  • WJPPS Impact Factor has been Increased to 8.025 for Year 2024.

  • WJPPS: MARCH ISSUE PUBLISHED
  • March Issue has been successfully launched on 1 March 2024.

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • Scope Indexed
  • WJPPS is indexed in Scope Database based on the recommendation of the Content Selection Committee (CSC).

Abstract

A NEW VALIDATED RP-HPLC METHOD FOR THE DETERMINATION OF GLYCOPYRROLATE AND FORMETEROL FUMARATE IN ITS BULK AND PHARMACEUTICAL DOSAGE FORMS

N. Md. Akram* and M. Umamahesh

ABSTRACT

A New method was established for simultaneous estimation of Glycopyrrolate and Formoterol fumarate by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Glycopyrrolate and Formoterol fumarate using Xterra column (4.6×150mm) 5μ, flow rate was 1ml/min, mobile phase ratio (40:60v/v) Methanol,1ml of OPA in 1000ml waterpH 3 (pH adjusted with triethylamine), detection wavelength used by WATERS HPLC Auto Sampler, Separation module 2695, UV detector 2489, Empower-software version-2. The retention times were found to be 3.0 mins and 4.20mins. The % purity of Glycopyrrolate and Formoterol fumarate were found to be 99.80 and 99.70 respectively. The present analytical method was validated according to ICH guidelines (ICH, Q2 (R1)). The linearity study of Glycopyrrolate and Formoterol fumarate was found in the concentration range 9μg/ml-45μg/mland 4.8μg/ml-24μg/ml and correlation coefficient (R2) be 0.999 and 0.999, % recovery was found to be 100.01 and 100.34, % RSD for repeatability 0.3 and 0.6, % RSD for intermediate precision was 0.3 and 0.4respectively. The precision study was precision, robustness and repeatability. It is a convenient, simple and quick method for the determination of Glycopyrrolate and Formoterol fumarate its bulk and pharmaceutical dosage forms.

Keywords: Glycopyrrolate, Formoterol fumarate, HPLC, Methanol.


[Download Article]     [Download Certifiate]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More