WJPPS Citation

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • New Impact Factor
  • WJPPS Impact Factor has been Increased to 8.025 for Year 2024.

  • WJPPS: MARCH ISSUE PUBLISHED
  • March Issue has been successfully launched on 1 March 2024.

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • Scope Indexed
  • WJPPS is indexed in Scope Database based on the recommendation of the Content Selection Committee (CSC).

Abstract

PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTICANCER STUDY OF ETHANOLIC EXTRACT OF LEAVES OF AMARANTHUS CRUENTUS LINN. AGAINST COLON CANCER CELL LINE (HCT-116)

*Vithya Sekar and N. Jayshree

ABSTRACT

Objective: The study is aimed at evaluating the anticancer activity of ethanolic extract of leaves of Amaranthus cruentus Linn. against colon cancer cell lines on the basis of its indigenous use and constituents. Method: The ethanolic extract of the leaves of Amaranthus cruentus prepared by using the soxhlet extraction method was subjected to phytochemical analysis to detect the plethora of secondary metabolities present. The total flavonoid content was determined using aluminium chloride colorimeteric method. The in vitro anticancer activity was studied using MTT assay against HCT – 116 (Colon cancer cell line).
The toxicity study against normal Vero cell line was also done to determine the selectivity of the extract for inhibiting cancer cells. 5-Fluorouracil was used as standard as it is the first choice of drug for the treatment of colon cancer. Results: The ethanolic extract showed presence of variety of secondary metabolites such as flavonoids, alkaloids, phenols, saponins, steroids and proteins. The IC50 value of EEAC was 138±4.24 μg/ml and that of 5-fluorouracil was 32.14±1.58 μg/ml against HCT-116. This indicates that the EEAC has cytotoxic effects comparable with 5-Fluorouracil. The IC50 value of EEAC was 1777±78.25μg/ml and that of 5-fluorouracil was 134.4±5.30 μg/ml against Vero cell line. This showed that the plant produce lesser cytotoxic damage to normal cells compared to standard 5 – Fluorouracil.

Keywords: MTT assay, Vero cell line, HCT-116, Amaranthus cruentus.


[Download Article]     [Download Certifiate]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More