Abstract

PROTECTIVE EFFECT OF NIGELLA SATIVA L. ON D-GALN/LPS INDUCED TOXICITY AND ITS CONSTITUENTS ANALYSIS BY HPLC TECHNIQUE

Dr. A. Mohamed Saleem Gani* and Dr. S. Ahmed John

ABSTRACT

The present work has been carried out to study the protective effect of alcoholic extract of Nigella sativa L. seeds (NSE) on D-Galactosamine (D-GalN) / Lipo polysaccharide (LPS) induced toxicity in rats and to analyze the constituents of the extract using HPLC technique. Twenty four adult wistar albino rats were divided into four groups viz Control, D-GalN/LPS induced toxicity, Nigella sativa alcoholic extract (NSE) treated and Nigella Sativa alcoholic extract pretreatment prior to the administration of D-GalN/LPS. After treatment, the rats were sacrificed, blood samples were collected for determination of serum high density lipo protein (HDL), low density lipoprotein (LDL), and very low density lipoprotein (VLDL). Levels of total cholesterol, triglycerides (TG) and phospholipids were analyzed from serum and liver samples. Level of total lipid analyzed from liver sample. Mean LDL, VLDL, TG, total cholesterol and total lipid were found to be significantly higher in D-GalN/LPS induced hepatotoxic rats group (P<0.05), mean HDL and phospholipid levels were found to be significantly decreased in D-GalN/LPS induced hepatotoxic rats group (P<0.05) than in the control group. The HPLC analysis confirmed the presence of various groups of secondary metabolites such as sterols, alkaloids and quinones. Levels of various parameters in the NSE pretreated group were found to be nearer to control and the constituents of the seed were found to be antioxidants which protected rats from D-GalN/LPS induced toxicity. Results of the present study strongly indicate that NSE having protective efficiency againt D-GalN/LPS induced toxicity in rats.

Keywords: Nigella sativa L., D-Galactosamine, Lipopolysaccharide, HPLC, Lipid profile.